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total extracts drg  (Proteintech)


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    Structured Review

    Proteintech total extracts drg
    List of primary antibodies
    Total Extracts Drg, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total extracts drg/product/Proteintech
    Average 93 stars, based on 15 article reviews
    total extracts drg - by Bioz Stars, 2026-02
    93/100 stars

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    1) Product Images from "Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features"

    Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

    Journal: Biology of Sex Differences

    doi: 10.1186/s13293-018-0164-z

    List of primary antibodies
    Figure Legend Snippet: List of primary antibodies

    Techniques Used: Isolation

    Effect of short-term diabetes on KIF5A. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of KIF5A. b KIF5A protein levels analyzed by immunoblotting in DRG total extracts of Ctrl and STZ M and F rats. c KIF5A protein levels analyzed by immunoblotting in isolated axoplasm of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05; ### p < 0.001) followed by Bonferroni post hoc test (significance: * p < 0.05)
    Figure Legend Snippet: Effect of short-term diabetes on KIF5A. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of KIF5A. b KIF5A protein levels analyzed by immunoblotting in DRG total extracts of Ctrl and STZ M and F rats. c KIF5A protein levels analyzed by immunoblotting in isolated axoplasm of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05; ### p < 0.001) followed by Bonferroni post hoc test (significance: * p < 0.05)

    Techniques Used: Reverse Transcription Polymerase Chain Reaction, Western Blot, Isolation



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    List of primary antibodies

    Journal: Biology of Sex Differences

    Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

    doi: 10.1186/s13293-018-0164-z

    Figure Lengend Snippet: List of primary antibodies

    Article Snippet: KIF5A , Total Extracts DRG , 1:1.000 , Proteintech (21186-1-AP).

    Techniques: Isolation

    Effect of short-term diabetes on KIF5A. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of KIF5A. b KIF5A protein levels analyzed by immunoblotting in DRG total extracts of Ctrl and STZ M and F rats. c KIF5A protein levels analyzed by immunoblotting in isolated axoplasm of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05; ### p < 0.001) followed by Bonferroni post hoc test (significance: * p < 0.05)

    Journal: Biology of Sex Differences

    Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

    doi: 10.1186/s13293-018-0164-z

    Figure Lengend Snippet: Effect of short-term diabetes on KIF5A. a RNA was extracted from DRG of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. RT-PCR was used to analyze mRNA levels of KIF5A. b KIF5A protein levels analyzed by immunoblotting in DRG total extracts of Ctrl and STZ M and F rats. c KIF5A protein levels analyzed by immunoblotting in isolated axoplasm of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed, and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05; ### p < 0.001) followed by Bonferroni post hoc test (significance: * p < 0.05)

    Article Snippet: KIF5A , Total Extracts DRG , 1:1.000 , Proteintech (21186-1-AP).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot, Isolation

    List of primary antibodies

    Journal: Biology of Sex Differences

    Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

    doi: 10.1186/s13293-018-0164-z

    Figure Lengend Snippet: List of primary antibodies

    Article Snippet: DRP1 , Total Extracts DRG , 1:500 , Cell signaling (14647S).

    Techniques: Isolation

    Effect of short-term diabetes on proteins involved in mitochondrial fission/fusion: MFN2, Drp1 and OPA1. a Immunoblot of DRP1 phosphorylation from DRG total extracts of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. The bar graph represents densitometric analysis and statistical evaluation of DRP1 phosphorylation over total DRP1 (DRP1P/DRP1 tot); representative western blot of phosphorylated and total DRP1 is presented above the graph. b Immunoblot of MFN2 from DRG total extracts of Ctrl and STZ M and F rats. c Immunoblot of OPA1 from DRG total extracts of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05) followed by Bonferroni post hoc test (significance: * p < 0.05)

    Journal: Biology of Sex Differences

    Article Title: Axonal transport in a peripheral diabetic neuropathy model: sex-dimorphic features

    doi: 10.1186/s13293-018-0164-z

    Figure Lengend Snippet: Effect of short-term diabetes on proteins involved in mitochondrial fission/fusion: MFN2, Drp1 and OPA1. a Immunoblot of DRP1 phosphorylation from DRG total extracts of control (Ctrl) and diabetic (streptozotocin, STZ) male (M) and female (F) rats. The bar graph represents densitometric analysis and statistical evaluation of DRP1 phosphorylation over total DRP1 (DRP1P/DRP1 tot); representative western blot of phosphorylated and total DRP1 is presented above the graph. b Immunoblot of MFN2 from DRG total extracts of Ctrl and STZ M and F rats. c Immunoblot of OPA1 from DRG total extracts of Ctrl and STZ M and F rats. Representative Western blots are presented above the graphs, with the respective loading controls (GAPDH). The densitometry of each band was analyzed and the results are expressed as percentage of M Ctrl. The columns represent the mean ± SEM, n = 6 animals for each group. The effects of sex, diabetes, and the interaction sex by diabetes were analyzed using two-way ANOVA (significance: # p < 0.05) followed by Bonferroni post hoc test (significance: * p < 0.05)

    Article Snippet: DRP1 , Total Extracts DRG , 1:500 , Cell signaling (14647S).

    Techniques: Western Blot